Figure 6.

Qualitative real-time polymerase chain reaction results of the last (fifth) week of CUS showing differential expression of selected four reference genes in adult zebrafish brain samples with significant expression differences detected previously by the RNA-seq. Data is analyzed and represented using the Pfaffl method. *p < 0.05, **p < 0.01, ***p < 0.001 vs. controls or vs. the group connected by a horizontal line, post-hoc Dunn’s test for pairwise comparisons for significant Kruskal–Wallis data (see Supplementary Table S10 for details). Graphs were constructed using the ggplot2 R package¹³⁷.