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. 2020 Nov 17;10:19959. doi: 10.1038/s41598-020-77004-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Western blot analysis for freshly-isolated cells: Proteins of INHBA, INHBB, and follistatin (FST) were detected from protein extracts (30 μg/lane). β-actin was used as an internal control by re-probing. Brain protein extracts, positive control of INHBA and INHBB; liver protein extracts, positive control of FST; SDS buffer, negative control. Typical blotting images are shown from 3 independent experiments. One set of experiments was performed using protein extracts of CD49f⁺ and CD49f^- cells fractionated from the salivary glands of 3 mice, and 3 sets of experiments carried out independently. (B) Densitometric analysis for protein levels of INHBA, INHBB, and FST. Signals were normalized to β-actin. One set of experiments was performed using protein extracts of CD49f⁺ and CD49f^- cells fractionated from the salivary glands of 3 mice, and 3 sets of experiments carried out independently. *P < 0.05, Student’s t test.