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. 2020 Nov 17;10:19959. doi: 10.1038/s41598-020-77004-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of external FST in CD49f⁺ cells after knockdown of Fst. CD49f⁺ cells transfected with Fst siRNA were treated with recombinant mouse Follistatin (rmFST: 0.2 μg/mL). Non target siRNA was also used as a negative control. After specific periods of rmFST addition, the total RNA was recovered and used for RT-qPCR analysis. Levels of (A) P21, (B) Cyclin D2, and (C) Pcna mRNA expression normalized to β-Actin are shown. One experiment was performed using sub-cultured CD49f⁺ cells fractionated from the salivary glands of 3 mice, and 3 independent experiments were carried out. *P < 0.05, one-way ANOVA and Tukey’s HSD test.