Figure 7.

Influence of DEFB118 on E. coli K88-induced inflammatory responses in IPEC-J2 cells. Total RNA was extracted from IPEC-J2 cells, and the expression of related genes was measured by real-time fluorescence PCR. The target gene mRNA expression level was calculated using the 2^–ΔΔCt method: (a) proinflammatory cytokine; (b) apoptotic factor. (A, B) Values within a column differ if they do not share a common superscript (P < 0.05). IL-1β: interleukin 1 beta; IL-6: interleukin 6; TNFα: tumor necrosis factor alpha.