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. 2020 Nov 3;23(1):18. doi: 10.3892/mmr.2020.11656

Figure 3.

Figure 3.

miR-325-3p negatively interacts with MEG3 in hypoxia-treated H9c2 cells. (A) The complementary binding sites between MEG3 and miR-325-3p, as well as the mutant sequences of MEG3. (B) The luciferase activities of WT-MEG3 and MUT-MEG3 in hypoxia-treated H9c2 cells transfected with miR-325-3p or miR-NC. (C) miR-325-3p expression levels in H9c2 cells after hypoxia treatment for 24, 48 or 72 h. (D) MEG3 expression levels in hypoxia-treated H9c2 cells transfected with vector or MEG3. (E) miR-325-3p expression levels in hypoxia-treated H9c2 cells transfected with si-NC, si-MEG3, vector or MEG3. n=3. *P<0.05. miR, microRNA; MEG3, maternally expressed 3; WT, wild-type; MUT, mutant; NC, negative control; si, small interfering RNA.