Figure 4.

miR-325-3p overexpression reduces hypoxia-induced injury in H9c2 cells by downregulating MEG3. H9c2 cells were transfected with miR-NC, miR-325-3p, miR-325-3p + vector or miR-325-3p + MEG3, and then cultured in hypoxic conditions. (A) miR-325-3p expression levels following transfection. (B) Cell viability was evaluated by performing an MTT assay. (C) Cell apoptosis was assessed via flow cytometry. (D) Protein expression levels of Bax, Bcl-2 and cleaved caspase-3 were detected via western blotting. (E) LDH release was measured by performing the LDH determination assay. n=3. *P<0.05. miR, microRNA; MEG3, maternally expressed 3; NC, negative control; LDH, lactate dehydrogenase; OD, optical density.