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. 2020 Nov 3;23(1):14. doi: 10.3892/mmr.2020.11652

Figure 2.

Figure 2.

miR-449a-5p inhibits neonatal CM proliferation. Isolated P1 CMs were transfected with mimic-NCs or mimic-miR-499a-5p. (A) Reverse transcription-quantitative PCR results for the detection of miR-449a-5p expression (n=3). (B) Ki67 immunofluorescence staining and quantification of ki67-positive CMs (n=3). Ki67-positive CMs were indicated by arrows. Scale bar, 50 µm. (C) pH3 immunofluorescence staining and quantification of pH3-positive CMs (n=3). pH3-positive CMs were indicated by arrows. Scale bar, 100 µm (upper images) or 20 µm (lower image). (D) Quantification of the number of CMs (n=3). Statistical significance was calculated using two-tailed unpaired Student's t-test. Error bars represent mean ± SEM. *P<0.05 vs. indicated groups. miR, microRNA; CM, cardiomyocyte; NC, negative control; cTnT, cardiac troponin T; pH3, histone H3 phosphorylated at serine 10.