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. 2020 Nov 3;23(1):14. doi: 10.3892/mmr.2020.11652

Figure 4.

Figure 4.

CDK6 is a target gene of miR-449a-5p. (A) Binding sites for miR-449a-5p on the 3′-UTR of CDK6 as predicted using miRmap and miRanda. (B) Luciferase assays of isolated P1 CMs transfected with luciferase-CDK6-WT, luciferase-CDK6-MU site 1 and luciferase-CDK6-MU site 2 (n=3). (C) Reverse transcription-quantitative PCR assays detected CDK6 mRNA expression in isolated P1 CMs transfected with mimic-NC, mimic-miR-449a-5p, inhibitor-NC or inhibitor-miR-449a-5p (n=3). (D) Western blotting results and semi-quantitative analyses of CDK6 protein expression in isolated P1 CMs transfected with mimic-NC, mimic-miR-449a-5p, inhibitor-NC or inhibitor-miR-449a-5p (n=3). Statistical significance was calculated using one-way ANOVA followed by Tukey's test in (B) or Least Significant Difference post hoc test in (C and D) Error bars represent mean ± SEM. *P<0.05 vs. indicated groups. miR, microRNA; UTR, untranslated region; P, postnatal; CMs, cardiomyocytes; WT, wild-type; MU, mutant; NC, negative control.