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. 2020 Nov 3;23(1):14. doi: 10.3892/mmr.2020.11652

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — CDK6 is involved in the inhibitory effects of miR-449a-5p on CM proliferation. P1 CMs were transfected with NC, mimic-miR-449a-5p or mimic-miR-449a-5p + pcDNA-CDK6 (n=3/group). (A) Reverse transcription-quantitative PCR results for the detection of CDK6 mRNA expression. (B) Western blotting results and semi-quantitative analyses of CDK6 protein expression. (C) Ki67 immunofluorescence staining and quantification of ki67-positive CMs. Ki67-positive CMs were indicated by arrows. Scale bar, 50 µm. Statistical significance was calculated using one-way ANOVA followed by Least Significant Difference post hoc test in A-C. Error bars represent mean ± SEM. *P<0.05 vs. indicated groups. miR, microRNA; CM, cardiomyocyte; P, postnatal; NC, negative control; cTnT, cardiac troponin T.