Table I.
Primer | Forward (5′→3′) | Reverse (5′→3′) |
---|---|---|
miR-449a-5p reverse transcription primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACCAGC | |
miR-449a-5p | CGCGCGTGGCAGTGTATTGTTA | ATCCAGTGCAGGGTCCGAGG |
U6 | CTCGCTTCGGCAGCACA | AACGCTTCACGAATTTGCGT |
CDK6 | TAGCTGTCTCCACCACCCAC | GGCCATCTGTCGTTAGCCAG |
RUNX1 | AACCAGGTAGCGAGATTCAACGAC | CAACTTGTGGCGGATTTGTAAAGA |
c-kit | CGGGCTAGCCAGAGACATCA | TCTCTGGTGCCATCCACTTCA |
DAB2 | TAGTCCAACAGAAAGCAAAG | GAGGTGACTCCATTTGTTAAG |
β-actin | TGCTGTCCCTGTATGCCTCTG | TTGATGTCACGCACGATTTCC |
miR, microRNA; RUNX1, Runt-related transcription factor 1; c-kit, mast/stem cell growth factor receptor kit; DAB2, disabled homolog 2.