Figure 3.

Overexpression of miR-26a-5p decreases lung tissue cell apoptosis in LPS-induced acute lung injury mice. Mice in each group were intraperitoneal injection of miR-26a-5p mimics or NC mimics (20 mg/kg) 24 h before treatment with 5 mg/kg LPS. And mice were sacrificed after LPS administration for 24 h and then lung tissues were collected for analysis. (A) Apoptotic cells in the lung tissues were detected by TUNEL staining (magnification, ×400) (n=5/group). The mRNA expression of (B) Bcl-2, (C) Bax and (D) cleaved caspase-3 in the lung tissues was detected by reverse transcription-quantitative PCR (n=5/group). (E) Representative western blot bands of Bcl-2, Bax and cleaved caspase-3. (F) Semi-quantitative data for the level of (F) Bcl-2, (G) Bax and (H) cleaved caspase-3 expression (n=5/group). Data were presented as the mean ± SD. **P<0.01 vs. Sham group; ^##P<0.01 vs. LPS + mimic NC group. miR, microRNA; NC, negative control; LPS, lipopolysaccharide; TUNEL, Terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling.