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. 2020 Nov 3;23(1):11. doi: 10.3892/mmr.2020.11649

Figure 2.

Figure 2.

Knockdown of LAMA3 inhibits PDAC cell proliferation, migration, invasion and EMT, and promotes PDAC cell apoptosis. (A) LAMA3 mRNA expression was evaluated via reverse transcription-quantitative PCR in PANC-1 cells transfected with si-LAMA3. (B) Cell proliferation was analyzed by Cell Counting Kit-8 assays in the Control, si-NC and si-LAMA3 groups. (C) Colony formation assays were performed in the si-NC and si-LAMA3 groups. (D) Transwell invasion and migration assays. (E) EMT-associated proteins E-cadherin and vimentin were examined via WB analysis in the Control, si-NC and si-LAMA3 groups. (F) The percentage of apoptotic cells was demonstrated by flow cytometry in the si-NC and si-LAMA3 groups. Scale bar, 100 µm. ***P<0.01 vs. si-NC group. PDAC, pancreatic ductal adenocarcinoma; EMT, epithelial-to-mesenchymal transition; siRNA, small interfering RNA; NC, negative control; LAMA3, laminin subunit α3; WB, western blotting.