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. 2020 Nov 3;23(1):11. doi: 10.3892/mmr.2020.11649

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Copyright: © Huang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Knockdown of LAMB3 inhibits PDAC cell proliferation, migration, invasion and EMT, and promotes PDAC cell apoptosis. (A) LAMB3 mRNA expression was evaluated via reverse transcription-quantitative PCR in PANC-1 cells transfected with si-LAMB3. (B) Cell proliferation was analyzed by Cell Counting Kit-8 assays in the Control, si-NC and si-LAMB3 groups. (C) Colony formation assays were performed in the si-NC and si-LAMB3 groups. (D) Transwell invasion and migration assays. (E) EMT-associated proteins E-cadherin and vimentin were examined via WB analysis in the Control, si-NC and si-LAMB3 groups. (F) The percentage of apoptotic cells was shown by flow cytometry in the si-NC and si-LAMB3 groups. *P<0.05, ***P<0.01 vs. si-NC group. PDAC, pancreatic ductal adenocarcinoma; EMT, epithelial-to-mesenchymal transition; siRNA, small interfering RNA; NC, negative control; LAMB3, laminin subunit β3; WB, western blotting.