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. 2020 Nov 3;23(1):23. doi: 10.3892/mmr.2020.11661

Figure 1.

Figure 1.

CUL4A is highly expressed in EC tissues and cell lines. (A) TCGA database was used to analyze the mRNA expression level of PITPNA-AS1 in NSCLC tumor tissues and adjacent non-tumor tissues. **P<0.01 vs. normal. CUL4A expression in EC cell lines (Ishikawa, KLE, RL95-2 and AN3CA) and normal human endometrial epithelial cells (hEEC) was examined via (B) RT-qPCR and (C) western blotting. *P<0.05, **P<0.01 and ***P<0.001 vs. hEEC. CUL4A expression following transfection with shRNA-CUL4A-1 or shRNA-CUL4A-2 was detected via (D) RT-qPCR and (E) western blotting. *P<0.05, **P<0.01 and ***P<0.001 vs. shRNA-NC. CUL4A, cullin 4A; EC, endometrial cancer; TCGA, The Cancer Genome Atlas; NSCLC, non-small cell lung cancer; RT-qPCR, reverse transcription-quantitative PCR; shRNA, short hairpin RNA; NC, negative control.