Figure 10.

Possible regulatory mechanisms between miR-218 and PTEN, YY1, BCL-2 and BMI-1 in NSCLC cells transfected with pGpU6/EGFP/Neo-miR-218. At the post-transcriptional level, PTEN, YY1, BCL-2 and BMI-1 expression levels were regulated by miR-218. Overexpression of miR-218 activated PTEN and YY1, and repressed BCL-2 and BMI-1 at the mRNA and protein levels. PTEN, YY1, BCL-2 and BMI-1 may have single or combined effects on NSCLC cell proliferation, migration, invasion and apoptosis at the mRNA and protein levels. miR, microRNA; NSCLC, non-small cell lung cancer; PTEN, anti-phosphatase and tensin homolog; BMI-1, anti-polycomb complex protein BMI-1; YY1, anti-transcriptional repressor protein YY1; VEGF, vascular endothelial growth factor.