Figure 8.

Overexpression of miR-218 inhibits the invasion of NCI-H157 and XWLC-05 cells. The number of invasive cells was determined by Transwell invasion assays and counted under an inverted microscope. Invasive cells were stained with 0.2% crystal violet and appear purple. The average number of invasive cells was calculated from 5 random fields. (A) Representative images of each group (magnification, ×100; scale bar, 20 µm). (B and C) Statistical calculations. Negative, negative control (transfected with pGpU6/EGFP/Neo-miR-NC plasmid) and data represent mean ± SD of three independent repeats. The experiment was repeated three times (n=3, **P<0.01, ***P<0.001 vs. control or negative control). miR, microRNA.