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. 2020 Nov 3;23(1):22. doi: 10.3892/mmr.2020.11660

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Copyright: © Kim et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Upregulation of TNF-α mRNA expression and protein levels in irradiated mIMCD-3 cells, and renal tissues of BALB/c and C57BL6 mice. (A) mIMCD-3 cells, and (B) BALB/c and C57BL/6 mice were treated with or without 6 Gy radiation. After 24 and/or 72 h, reverse transcription-quantitative PCR was applied to assess TNF-α mRNA expression using GAPDH as the loading or internal control. ELISA was conducted to measure (C) protein levels of TNF-α (pg/ml) in mIMCD-3 cells, and (D) fold-change values of TNF-α levels in BALB/c and C57BL/6 mice relative to the control group. Data are presented as the mean ± SD (n=4). *P<0.05, **P<0.01 vs. Control. mIMCD-3, mouse inner medullary collecting duct-3; IR, ionizing radiation.