. 2020 Nov 18;27:62. doi: 10.1051/parasite/2020058

Table 1.

Primer sequences and reaction conditions used in nested PCR amplifications.

Locus	Primer sequences (5′ – 3′)	Nucleotide fragment (bp)	Annealing temperature (°C)	Reference
Cryptosporidium spp. SSU rRNA	SSU-F2: TTCTAGAGCTAATACATGCG	~1325	55	Xiao et al. [37]
	SSU-R2: CCCATTTCCTTCGAAACAGGA	~1325	55
	SSU-F3: GGAAGGGTTGTATTTATTAGATAAAG	826–864	55
	SSU-R4: CTCATAAGGTGCTGAAGGAGTA	826–864	55
C. parvum gp60	AL3531: ATAGTCTCCGCTGTATTC	1280	52	Alves et al. [1]
	AL3535: GGAAGGAACGATGTATCT	1280	52
	AL3532: TCCGCTGTATTCTCAGCC	800–850	50
	AL3534: GCAGAGGAACCAGCATC	800–850	50
G. duodenalis SSU rRNA	Gia2029: AAGTGTGGTGCAGACGGACTC	497	55	Appelbee et al. [2]
	Gia2150c: CTGCTGCCGTCCTTGGATGT	497	55
	RH11: CATCCGGTCGATCCTGCC	292	59
	RH4: AGTCGAACCCTGATTCTCCGCCCAGG	292	59
G. duodenalis tpi	AL3543: AAATIATGCCTGCTCGTCG	605	50	Cacciò and Ryan [4]
	AL3546: CAAACCTTITCCGCAAACC	605	50
	AL3544: CCCTTCATCGGIGGTAACTT	530	50
	AL3545: GTGGCCACCACICCCGTGCC	530	50