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. 2020 Nov 12;9(11):bio057174. doi: 10.1242/bio.057174

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© 2020. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Haploid and diploid HAP1 cells can be distinguished based on PI stain in a flow cytometer. (A) Ploidy-verified reference cells (HAP1 C631 haploid and diploid) were fixed, DNA-stained with PI and analyzed on a BD Accuri C6. Linear plots of fluorescence intensity height (FL2-H) against total cell fluorescence (FL2-A) illustrate the difference in DNA-quantity between haploid (orange) and diploid (red) HAP1 cells. (B) Example result of ploidy test showing flow data for the cell line ‘Gene specific KO-A’ at passage 28, referenced to ploidy-verified control lines. Histograms show number of cells against intensity of the DNA. Fluorescence intensity is shown in linear scale (both A and B).