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. 2020 Nov 12;9(11):bio057174. doi: 10.1242/bio.057174

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© 2020. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Confocal imaging of haploid and diploid HAP1 cells showed large ploidy-dependent differences. Ploidy-verified haploid and diploid HAP1 cells subjected to immunocytochemistry and imaged with a confocal microscope. (A) Overview of cell culture as observed with 40× objective (left) and 100×objective (right). Nuclei were visualized with DAPI and F-actin with fluorescent phalloidin. (B) A closer look at structures in single cells (3×optical zoom, 100×objective). Nuclei and F-actin were stained as in A. In addition, mitochondria were visualized with antibody towards Cox IV and microtubules with antibody towards β-tubulin.