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. 2020 Nov 10;223(21):jeb233197. doi: 10.1242/jeb.233197

Fig. 2.

Fig. 2.

Marker proteins used to examine polarity in LWamide-like+ Nematostella neurons. (A) C-terminal fusion of GFP to NvEB1 labels the plus end of microtubules in the GTP-tubulin cap region. The γ-tubulin ring complex (γ) nucleates microtubules and can cap the minus end. This region moves over time as microtubules polymerize (arrow). (B) C-terminal fusion of Citrine to mCD8 makes a transmembrane fluorescent marker labeled on the cytoplasmic side. The marker is not tethered and can diffuse in the plane of the membrane. (C) C-terminal fusion of mCherry to NvSynaptobrevin, the synaptic vesicle SNARE protein and Emerald to the LWamide-like peptide precursor label synaptic vesicles to mark presynaptic release sites. (D) C-terminal fusion of GFP to NvHomer labels the postsynaptic scaffold network formed by Homer and Shank. The identity of receptor targets of Homer at Nematostella synapses is unknown.