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. 2020 Nov 12;9(11):bio053447. doi: 10.1242/bio.053447

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© 2020. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — MIF promoted proliferation of BPH-1 and PWR-1E cells. (A) MTT assay showed the proliferation of BPH-1 cells treated with various concentrations of rMIF for 3 days. Data are presented as mean±s.d., n=3. (B) CCK8 assay showed the proliferation of BPH-1 cells treated with control, rMIF (100 ng/ml), rMIF (100 ng/ml)+ISO-1 (10 µM) and ISO-1 (10 µM), respectively. Data are presented as mean±s.d., n=3. (C) CCK8 assay showed the proliferation of PWR-1E cells treated with control, rMIF, rMIF+ISO-1 and ISO-1, respectively. Data are presented as mean±s.d., n=3. (D) JC-1 assay showed the growth rates of the BPH-1 cells treated by control, rMIF, rMIF+ISO-1 and ISO-1, respectively. Data are presented as mean±s.d., n=3. (E) JC-1 assay showed the growth rates of the PWR-1E cells treated by control, rMIF, rMIF+ISO-1 and ISO-1, respectively. Data are presented as mean±s.d., n=3. *P<0.05, *P<0.05. Statistical analyses were performed using ANOVA followed by Tukey's test for multiple comparison.