Figure 7.

Nitric oxide promotes the apoptosis of activated neutrophil (A) The hepatic cell numbers of CD11b⁺Ly6G⁺ neutrophil at 6 h after acetaminophen (APAP) injection (n=6–8). *P < 0.05, ***P < 0.001, NS, no significance, Kruskal-Wallis test. (B) Total ROS/RNS levels in liver tissue are represented by DCF concentration. *P < 0.05, **P < 0.01, NS, no significance, Kruskal-Wallis test. (C) Hepatic neutrophils from the mice injected with APAP alone or combined with adoptive transfer of TNF-α/lipopolysaccharide (LPS) MDSC were isolated to measure the levels of elastase (n=6 per group). Naïve neutrophils were the blood neutrophils of healthy mice (n=3). *P < 0.05, NS, no significance, Kruskal-Wallis test. For neutrophil apoptosis assessment, 1×10⁵ neutrophils were cultured with MDSCs in equal numbers (D) or DPTA (E) for 3 h (n=9 per group). 50nM of PMA was added for neutrophil activation. The apoptosis of neutrophil was analyzed by flow cytometry. *P < 0.05, **P < 0.01, ***P < 0.001,. Kruskal-Wallis test. Data were expressed as mean ± SD.