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. 2020 Oct 28;11:590337. doi: 10.3389/fpls.2020.590337

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Copyright © 2020 Berka, Luklová, Dufková, Berková, Novák, Saiz-Fernández, Rashotte, Brzobohatý and Černý.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytokinin impact on the ROS production. The estimated mean content of hydrogen peroxide in barley roots determined by (A) PeroxiDetect Kit and (B) 3,3′-diaminobenzidine (DAB) staining; (C) Cytokinin impact on superoxide radical production as estimated by histochemical staining with nitroblue tetrazolium (NBT); Representative images of cytokinin- and mock-treated roots stained with DAB (D) and NBT (E). Presented data are means and standard deviation of at least three biological replicates; Statistically significant differences (Student’s t-test) are indicated.