In the pituitary of 24 and 48 h fasted mice, mRNA levels were quantified by qRT-PCR for (a) pituitary-specific positive transcription factor 1 (Pit1), (b) growth hormone (Gh), (c) prolactin (Prl), (d) luteinizing hormone (Lh), (e) thyroid stimulating hormone, and (f) follicle-stimulating hormone (Fsh). n = 4–8 per condition; error bars are SEM. Values that differ significantly (P < 0.05) with the same time point among different treatment conditions: “a” significant with fed group and “b” significant with fasted group. Student’s t-test.