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| Gene | Primer (5’–3’) | Product size (bp) |
|---|---|---|
| Oct4 | F: GTTCTCTTTGGAAAGGTGTTC R: ACACTCGGACCACGTCTTTC |
313 |
| Nanog | F: GTGTTTGGTGAACTCTCCTG R: GGGAATTGAAATACTTGACAG |
307 |
| Sox-2 | F: CATCCACAGCAAATGACAGC R: TTTCTGCAAAGCTCCTACCG |
251 |
| β-actin | F: GGAATCCTGTGGCATCCATGAAAC R: AAAACGCAGCTCAGTAACAGTCCG |
348 |
