| Reagents | 1 rxn (μL) | 12 rxns (μL) |
|---|---|---|
| Nuclease free water | To bring to 50 | To bring to 600 |
| Cut Smart buffer | 5 | 60 |
| VHH and VH PCR fragments | 1 μg | 12 μg |
| SfiI | 2 | 24 |
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