| Each experimental reaction | Negative control reaction | |
|---|---|---|
| ddH2O | To bring to 20 μL | To bring to 20 μL |
| 10X buffer | 2 | 2 |
| VHH and VH fragments | ≈ 53 μg | 0 μg |
| pComb3XSS | ≈ 150 μg | ≈ 150 μg |
| Ligase | 1 | 1 |
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