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. 2020 Nov 6;6(45):eabc4882. doi: 10.1126/sciadv.abc4882

Fig. 3. DEL-1 deficiency promotes melanoma lung metastasis in a neutrophil-dependent manner.

Fig. 3

(A) Representative lung images (top) and corresponding DsRed fluorescence images (bottom) showing the diminished metastases to the lungs of Del1KO mice (n = 5 per group) treated with anti-Ly6G antibody. Photo credit: Hyung-Joon Kwon, University of Ulsan. (B) Quantification of lung metastases by FLI depicted in (A). (C and D) Representative (C) and quantitative (D) flow cytometric analysis of myeloid cells and lymphocytes, graphed on Ly6C by Ly6G and CD3 by NK1.1 dot plots, respectively, among CD45+ cell populations in the metastasis-bearing lungs depicted in (A). (E and F) Representative (E) and quantitative (F) flow cytometric analysis of CD11b and CD27 expression on NK1.1+ NK cells depicted in (A). (G and H) Representative (G) and quantitative (H) flow cytometric analysis of intracellular IL-17A expression in different lymphocytes in the metastasis-bearing lung of WT and Del1KO mice (n = 6 per group). (I and J) Representative (I) and quantitative (J) flow cytometric analysis of intracellular IL-17A expression in different lymphocytes depicted in (A). Horizontal bars indicate the means (D, F, H, and J). *P < 0.05; **P < 0.01.