Fig. 4. Melanoma lung metastasis and neutrophil accumulation are ameliorated by Del-1-Fc administration.

(A and B) C57BL/6 mice were treated with intravenous injection of control-Fc or Del-1-Fc 6 hours before an intravenous injection of 2 × 10⁵ B16F10 cells expressing DsRed and twice a week treatment thereafter. (A) Representative lung images (top) and the corresponding DsRed fluorescence images (bottom) showing lung metastases (n = 8 each group) after 14 days of tumor implantation. Photo credit: Hyung-Joon Kwon, University of Ulsan. (B) Quantification of lung metastases by FLI depicted in (A). (C and D) Representative (C) and quantitative (D) flow cytometric analysis of different myeloid cells and lymphocytes, graphed on Ly6C by Ly6G and CD3 by NK1.1 dot plots, respectively, in the metastasis-bearing lungs depicted in (A). Horizontal bars indicate the means (D). *P < 0.05.