Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) | tdTomato | Jackson Laboratory | Stock #:007914 RRID:IMSR_JAX:007914 |
PMID:20023653 |
| Genetic reagent (M. musculus) | Gcg-Cre | PMID:28218622 | RRID:MMRRC_051056 | Dr. Michael Scott (University of Virginia) |
| Genetic reagent (M. musculus) | LepRb-Cre | Jackson Laboratory | Stock #:032457 RRID:IMSR_JAX:032457 |
Dr. Martin Myers, Jr. (University of Michigan) PMID:17021368 |
| Genetic reagent (M. musculus) | Lepob/ob | Jackson Laboratory | Stock #:000632 RRID:IMSR_JAX:000632 |
Lepob/+ heterozygotes were bred to generate homozygous, leptin-deficient mice |
| Genetic reagent (M. musculus) | LepRTB/TB | Jackson Laboratory | Stock #:018989 RRID:IMSR_JAX:018989 |
PMID:22326958 |
| Genetic reagent (M. musculus) | Glp1r-Cre | Jackson Laboratory | Stock #:029283 RRID:IMSR_JAX:029283 |
PMID:27238020 |
| Genetic reagent (M. musculus) | Synaptophysin-tdTomato | Jackson Laboratory | Stock #:012570 RRID:IMSR_JAX:012570 |
MGI ID: J:170755 |
| Antibody | Rabbit monoclonal anti-cFos | Cell Signaling | Cat. #: 2250S RRID:AB_2247211 |
IHC (1:1000) |
| Antibody | Rabbit polyclonal anti-CRF | Dr. Paul Sawchenko; Dr. Wylie Vale (Salk Institute) | PBL#rC68 | IHC (1:1000) |
| Antibody | Mouse monoclonal anti-HuC/D | Molecular Probes | Cat. #: A21271 RRID:AB_221448 |
IHC (1:500) |
| Antibody | Rabbit polyclonal anti-dsRed | Clontech | Cat. #: 632496 RRID:AB_10013483 |
IHC (1:1000) |
| Antibody | Rabbit anti-Oxytocin | Peninsula | Cat. #: T-4084 RRID:AB_518524 |
IHC (1:2500) |
| Antibody | Rabbit polyclonal anti-GLP-1 | Peninsula | Cat. #: T-4363 RRID:AB_518978 |
IHC (1:5000) |
| Antibody | Rabbit polyclonal anti-pSTAT3 (Y705) | Cell Signaling | Cat. #: 9131S RRID:AB_331586 |
IHC (1:1000) |
| Antibody | Goat polyclonal anti-rabbit Alexa Fluor 568 | ThermoFisher Scientific | Cat. #: A-11036 RRID:AB_10563566 |
IHC (1:500) |
| Antibody | Goat polyclonal anti-rabbit Alexa Fluor 488 | ThermoFisher Scientific | Cat. #: A-11034 RRID:AB_2576217 |
IHC (1:500) |
| Antibody | Goat polyclonal anti-mouse Alexa Fluor 647 | ThermoFisher Scientific | Cat. #: A-21236 RRID:AB_2535805 |
IHC (1:500) |
| Recombinant DNA reagent | AAV pCAG-FLEX-EGFP-WPRE | Addgene | Cat. #: 51502 RRID:Addgene_51502 |
PMID:24695228 |
| Peptide, recombinant protein | Leptin | Peprotech | Cat. #: 450–31 | 10 mg/kg i.p. |
| Peptide, recombinant protein | CCK | Bachem | Cat. #: H-2080 | 10 μg/kg i.p. |
| Commercial assay or kit | Corticosterone RIA | MP Biomedicals | Cat. #: 07–120102 | |
| Chemical compound, drug | Colchicine | Sigma | Cat. #: C9754 | 4 mg/mL |
| Software, algorithm | Imaris | Bitplane | V9.2 | |
| Software, algorithm | Volocity | PerkinElmer | V6.1.1 | |
| Software, algorithm | GraphPad Prism | Prism | Prism 7 |