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. 2020 Nov 2;9:e55547. doi: 10.7554/eLife.55547

Figure 5. WASp is necessary for mitochondrial homeostasis.

Figure 5.

(a) Representative images of different mitochondrial morphologies from healthy donor MDMs fixed and stained for mitochondria (biotin, green) and nuclei (DAPI, blue). Imaged by confocal microscopy at 63x. Scale bar = 10 µm. (b) Representative images of healthy donor (n = 7), WAS patient (n = 3) or WAS patient post gene therapy (n = 2) MDMs fixed and stained for mitochondria (biotin, green), f-actin (phalloidin, red) and nuclei (DAPI, blue). Imaged at 63x by confocal microscopy, with higher magnification of area inside white boxes displayed to the right. Scale bar = 10 µm. (c) Combined analysis from (b and d). Slides blinded prior to imaging and analysis. At least 100 cells per slide analysed for mitochondrial morphology and categorised according to appearance as indicated. Bars represent mean +/- SEM. (d) Representative images of healthy donor MDMs (n = 7), CK666 100µM-treated healthy donor MDMs (n = 2), or MDMs from ARPC1B-deficient patients (n = 3) fixed and stained for mitochondria (biotin, green) and nuclei (DAPI, blue). Imaged at 63x by confocal microscopy and higher magnification of area highlighted by white boxes shown to the right. Scale bar = 10 µm. DAPI, 4’,6-diamidino-2-phenylindole; HC, healthy control; MDM, monocyte-derived macrophage; SEM, standard error of mean; WAS, Wiskott Aldrich syndrome.