Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | WT THP-1 | ATCC | TIB-202 | Male, 1 year infant |
| Cell line (Homo sapiens) | WAS KO THP-1 | doi: 10.1038/s41467-017-01676-0 | Male, 1 year infant | |
| Antibody | Anti-human WASP (Mouse monoclonal) | BD Bioscience | Cat. #: 557773, RRID:AB_396867 | FACS (1:100), WB (1:500) |
| Antibody | Anti-mouse IgG Alexa Fluor 647 (Goat monoclonal) |
BioLegend | Cat. #: 405301, RRID:AB_315005 | FACS (1:200) |
| Antibody | Anti-human LAMP-1 (Mouse monoclonal) | BD Bioscience | Cat. #: 17–611043; RRID:AB_3983356 | WB (1 in 1000) |
| Antibody | Anti-human LC3B (Rabbit monoclonal) | Sigma | Cat. #: L7543; RRID:AB_796155 | WB (one in1000) |
| Antibody | Anti-human GAPDH (Mouse monoclonal) | Santa Cruz | Cat. #: sc365062; RRID:AB_10847862 | WB (1 in 1000) |
| Antibody | Anti-mouse IgG HRP (Sheep monoclonal) | GE Healthcare | Cat. #: NA9310-1ML; RRID:AB_772193 | WB (1 in 2000) |
| Antibody | Anti-rabbit IgG HRP (Donkey monoclonal) | GE Healthcare | Cat. #: NA9340-1ML; RRID:AB_772191 | WB (1 in 2000) |
| Antibody | Anti-human LC3 (Rabbit polyclonal) | MBL | Cat. #: PM036; RRID:AB_2274121 | IF (1 in 200) |
| Antibody | Anti-human LAMP-1 (Mouse monoclonal) | CST | Cat. #: 15665; RRID:AB_2798750 | IF (1 in 50) |
| Antibody | Anti-human Vinculin (Mouse monoclonal) | Sigma | Cat. #: V4505; RRID:AB_477617 | IF (1:200) |
| Antibody | Anti-mouse IgG Alexa Fluor 488 (Goat polyclonal) | Molecular Probes | Cat. #: A32723; RRID:AB_2633275 | IF (1 in 500) |
| Antibody | Anti-rabbit IgG Alexa Fluor 546 (Goat polyclonal) | Molecular Probes | Cat. #: A-11035; RRID:AB_143051 | IF (1 in 500) |
| Antibody | Anti-rabbit IgG Alexa Fluor 647 (Goat polyclonal) | Molecular Probes | Cat. #: A27040; RRID:AB_2536101 | IF (1 in 500) |
| peptide, recombinant protein | Fibronectin | R and D system | Cat. #: 4305-FNB-200 | |
| commercial assay or kit | CD14+ Microbead | Miltenyi Biotec | Cat. #: 130-050-201 | |
| Chemical compound, drug | Rapamycin | Calbiochem | Cat. #: 553211 | |
| Chemical compound, drug | Bafilomycin A1 | Sigma | Cat. #: SML1661 | |
| Chemical compound, drug | CK666 | Abcam | Cat. #: ab141231 | |
| Chemical compound, drug | CCCP | Merck | Cat. #: 215911 | |
| Chemical compound, drug | Oligomycin | Sigma | Cat. #: 75351 | |
| Chemical compound, drug | FCCP | Sigma | Cat. #: C2920 | |
| Chemical compound, drug | Rotenone | Sigma | Cat. #: R8875 | |
| Software, algorithm | ImageJ | NIH | RRID:SCR_003070 | |
| Software, algorithm | Prism | GraphPad | Version 8 | |
| Software, algorithm | iLIR | doi: 10.4161/auto.28260 | Version 1 | |
| Other | Prolong Diamond anti-fade mounting solution with DAPI | Molecular probes | Cat. #: P36962 | |
| Other | Phalloidin-633 | Molecular Probes | Cat. #: A22284 |