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. 2020 Nov 18;9:e56795. doi: 10.7554/eLife.56795

Figure 2. Development of FS interneurons in the mouse mPFC.

(a) Left, examples of PV immunostaining in the mPFC at different ages. Right, scatter plot displaying the density of PV-immunopositive neurons in the mPFC of P5-40 mice (n = 38 mice). (b) Same as (a) for SOM-immunopositive neurons (n = 39 mice). (c) Example mean waveforms of extracellular recorded single units from P5-40 mice. (d) Schematic showing features classically used to distinguish RS and FS units in adult mice. (e) Left, scatter plot showing the first two components of a t-sne dimensionality reduction on the mean waveforms for all units recorded from P5-40 mice (n = 3554 units from 66 recordings/mice). Right, same as left with the first two clusters obtained by hierarchical clustering labeled in black and red. (f) Scatter plot of half width and trough-to-peak time for cluster 1 (black) and 2 (red). (g) Mean waveform for cluster 1 (black) and 2 (red). (h) Scatter plot showing the proportion of FS units for P5-40 mice. (i) Scatter plots showing classic spike shape features for P5-40 for cluster 1 (RS, black) and 2 (FS, red). (See Supplementary file 1 for a summary of experimental conditions. See Supplementary file 2 for statistics).

Figure 2—source data 1. Source data for Figure 2a,b.
Figure 2—source data 2. Source data for Figure 2f.
Figure 2—source data 3. Source data for Figure 2g.
Figure 2—source data 4. Source data for Figure 2h.
Figure 2—source data 5. Source data for Figure 2i.

Figure 2.

Figure 2—figure supplement 1. t-sne dimensionality reduction.

Figure 2—figure supplement 1.

(a) Dendrogram of binary hierarchical clustering after t-sne dimensionality reduction. The height of the lines represents the distance between subclusters. (b) Scatter plot showing the first two components of a t-sne dimensionality reduction on the mean waveforms for all units recorded from P5-40 (n = 3554 units from 66 recordings/mice) color coded by age. (c) Same as (b) color coded by mPFC layer. (d) Same as (b) color coded by values of classic spike shape features (half width, trough to peak time, amplitude).
Figure 2—figure supplement 1—source data 1. Source data for Figure 2—figure supplement 1b–d.