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. 2020 Nov 18;6(47):eabb7135. doi: 10.1126/sciadv.abb7135

Fig. 2. LPL−/− promotes angiogenesis by increasing preosteoclasts.

Fig. 2

(A) TRAP staining images and quantitative analyses of preosteoclasts on the trabecular bone and periosteal bone surface of the femoral diaphysis. Arrowhead, multiple nuclear osteoclasts; arrow, TRAP+ cells. Scale bars, 50 μm. (B) Quantification of TRAP+ cells/bone surface (N. TRAP+ cells/B.Pm) and multiple nuclear osteoclast numbers/bone surface (N.Mu.Oc/B.Pm). n = 5 per group. (C) Quantification of preosteoclast/periosteal bone surface (N.POCs/BS). (D) Angiographic images of femora. Scale bar, 20 μm. (E) Quantitative analyses of vessel surface and vessel volume. n = 5 per group. (F) Representative images of immunostaining of endomucin (EMCN) (red), CD31 (green), and EmcnhiCD31hi (yellow) cells on the trabecular bone and periosteal bone. Scale bar, 50 μm. (G) The percentage of CD31hiEmcnhi area on the trabecular bone and periosteal bone. n = 5 per group. (H) Flow cytometry plots with the percentage of CD31hiEmcnhi endothelial cells (ECs) in total bone marrow cells. n = 3 per group. (I) Immunostaining of TRAP (red) and PDGF-BB (green) on the trabecular bone and periosteal bone. Scale bar, 50 μm. (J) Quantification of PDGF-BB+ and VEGF+ cell numbers per respective bone surface. n = 6 per group. (K) Serum PDGF-BB and VEGF levels. n = 6 per group. *P < 0.05, **P < 0.01, and ***P < 0.001).