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. 2020 Nov 18;6(47):eabc9920. doi: 10.1126/sciadv.abc9920

Fig. 3. VG3 amacrine cells provide excitatory synaptic input to W3 and OFFα ganglion cells.

Fig. 3

(A and B) Optogenetic stimulation of VG3 amacrine cells (VG3-Cre Ai32 mice) elicits postsynaptic currents in W3 (A; n = 7 cells) and OFFα (B; n = 5 cells) ganglion cells. Reversal near 0 mV identifies these as excitatory postsynaptic currents (EPSCs). (C and D) Overview projections (C) and single-plane excerpts (D) of a confocal image stack of an OFFα cell biolistically labeled with cyan fluorescent protein (CFP; C and D) and PSD95-YFP (D) in a retina, in which VG3 amacrine cells express tdTomato (VG3-Cre Ai9 mice). (E) Summary data of the fraction of PSD95-YFP puncta apposed by VG3 boutons in the obtained image stacks (observed) or the tdTomato channel was rotated by 90° (rotated, n = 6 cells; observed versus rotated: P = 0.03 by Wilcoxon signed-rank test). Exc, excitation; I, current; V, voltage.