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. 2020 Dec 1;11(6):1374–1394. doi: 10.14336/AD.2020.1020

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copyright: © 2020 Hattiangady et al.

this is an open access article distributed under the terms of the creative commons attribution license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 3. — Cells derived from neural stem cell (NSC) grafts displayed long-term survival and differentiated into all three neural cell types in the host hippocampus that underwent SE-induced injury. The top panel (A1-A3) shows the 5'-Chloro-2'-deoxyuridine CldU-positive graft-derived cells in the host hippocampus at ~9 months post-grafting (A1). A2 and A3 are magnified views of regions from A1, depicting the distribution of CldU+ cells in the graft core (A2) and neighboring dentate granule cell layer (GCL, A3). B1-C4 demonstrate samples of confocal Z section images visualizing graft-derived neurons positive for CldU and NeuN in the CA3 region (B1-B3) and the GCL (C1-C3). B4 and C4 show orthogonal views of graft-derived neurons expressing CldU-NeuN. The bar chart D depicts the overall neuronal differentiation of graft-derived cells in the GCL (neurogenic) and the graft core (D). Figures E1-E4 demonstrate graft-derived CldU+ cells expressing gamma-aminobutyric acid (GABA). E4 shows an orthogonal view of a graft-derived interneuron expressing CldU and GABA. The bar chart F depicts the overall % of graft-derived cells differentiating into GABA-ergic interneurons. Figures G1-I4 show the differentiation of graft-derived CldU+ cells into S-100ß+ mature astrocytes (G1-G3), NG2+ oligodendrocyte progenitors (G1-G3), and O4+ oligodendrocytes (I1-I3) in the host hippocampus. G4, H4, and I4 show the orthogonal view of a graft-derived astrocyte (G4), an oligodendrocyte progenitor cell (H4), and an oligodendrocyte (I4). The bar chart J shows the percentage of S-100ß+ astrocytes, NG2+ oligodendrocyte progenitors, and oligodendrocytes among graft-derived cells. Scale bars: A1, 200 µm; A2 and A3, 50 µm; B1-E4, G1-G3, H1-H3, and I1-I4, 10 µm; G4 and H4, 5 µm. DH, dentate hilus.