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Primer sequences for RT-PCR of target genes.
| Primer name | Amplicon length (bp) | Primer sequence | |
|---|---|---|---|
| β-Actin | 113 | Forward | CAGCCCTCCTTCATCGGTAT |
| Reverse | GACATGACGTTGTTGGCGTA | ||
|
| |||
| PI3Kp85α | 182 | Forward | AACCCTGCAGAACTACGACA |
| Reverse | GGCGCTCTGTATTTCTTGGG | ||
|
| |||
| PI3Kp110α | 182 | Forward | GAGGTTTGGCCTGCTTTTGG |
| Reverse | CTGGTTGCCTCATTTGCTCA | ||
|
| |||
| AKT2 | 132 | Forward | ACGCATTCCAGACCCATGAC |
| Reverse | AGACGATTTCCGCACCGTAG | ||
|
| |||
| AKT3 | 183 | Forward | GAGGACCGCACACGTTTCTA |
| Reverse | TGTCTTCATGGTGGCTGCAT | ||
