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. 2020 Nov 17;11:5863. doi: 10.1038/s41467-020-19570-7

Fig. 3. PrimPol-mediated re-priming promotes RAD51 loading in response to bulky DNA adducts.

Fig. 3

a RAD51 foci after 24 h release from 50 nM BPDE in the presence of nonT or PrimPol siRNA. Images are representative of n = 3. Scale bars: 10 μm. b Percentages of U2OS cells with >5 RAD51 foci after release from 50 nM BPDE, in presence of nonT or PrimPol siRNA. n = 3. c Representative cell cycle distributions after release from solvent (1 h) or 50 nM BPDE in presence of nonT or PrimPol siRNA. Cells were incubated with BrdU for 30 min to label replicating cells prior to flow cytometry. Percentages are means from three independent experiments as shown in Figure S4A. d Percentages of cells with >5 RAD51 foci after release from 50 nM BPDE, with or without PrimPol shRNA depletion and expression constructs encoding GFP or WT or CH PrimPol. The release from the solvent was 1 h. n = 3. Source data are provided as a Source Data file. The means and SEM (bars) of at least three independent experiments are shown. Asterisks indicate p-values (one-sided student’s t-test for B or one-way ANOVA for D, *p < 0.05, **p < 0.01).