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. 2020 Nov 17;11(11):988. doi: 10.1038/s41419-020-03194-2

Fig. 1. TalaA killed colorectal cancer cells.

Fig. 1

A The structure of TalaA. B CRC cells were incubated with TalaA in DMEM media containing 10% FBS for 24 h, then the CCK8 kit was employed to examine the cells activities. From left to right, the cells were HCT116, SW480, and SW620, respectively. For each concentration point, three repeats were performed. C CRC cells were incubated with TalaA in DMEM media containing 1% FBS for 24 h, then the CCK8 kit was employed to examine the cells activities. From left to right, the cells were HCT116, SW480, and SW620, respectively. For each concentration point, three repeats were performed. D After HCT116 cells were incubated with TalaA in 10% FBS contained media for 48 h, Edu solution was added and cells were stained according to manufacturer’s instruction. Red spots meant Edu-positive cells, and blue spots meant Hoechst33342-positive cells. E After SW480 cells were incubated with TalaA in 10% FBS contained media for 48 h, Edu solution was added and cells were stained according to manufacturer’s instruction. Red spots meant Edu-positive cells, and blue spots meant Hoechst33342-positive cells. F The crystal violet staining results for the clonogenicity of SW480 cells. The SW480 cells were cultured with 0–10 μM TalaA for 12 days.