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. 2020 Nov 17;10:20002. doi: 10.1038/s41598-020-77092-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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TMEM16A blocker MONNA suppresses arterial contraction stronger in 1- to 2-week-old rats in comparison with 2- to 3-month-old rats. (a-b) CRCs to methoxamine in the presence of vehicle (black symbols) or 3 μM MONNA (red symbols) of endothelium-denuded arteries from 2- to 3-month-old (a) and 1- to 2-week-old (b) rats. (c) Areas under individual CRCs to methoxamine in the presence of 3 μM MONNA in (a) and (b) expressed as percentage of mean value in the corresponding group exposed to vehicle. *p < 0.05 (Repeated measures ANOVA), # p < 0.05 (Student’s t-test). Numbers in parentheses indicate the number of rats.