Table A.3.
Studies that did not directly compare saliva use in SARS-CoV-2 detection with respiratory samples. Natal/RN, 2020.
| Authors | Study design | Study population | Sample size [Gender] | Age range [Mean] (Years) | Test | Saliva type | Saliva collection method | Another samples | Results |
|---|---|---|---|---|---|---|---|---|---|
| Zhang et al., 202044 | Cohort study | Patients at Wuhan Pulmonar Hospital. | 15 [-] | – | RT-qPCR | Saliva | Oral swab. | Anal swab and blood. | 8 patients (53.3%) were positive for 2019-nCoV in oral swab on day 0, while there were only 4 (25%) positive oral swab on day 5. |
| Hung et al., 202045 | Cohort study | Patients confirmed with SARS-CoV-2 infection. | 18 [8 M, 10 M] | 18-61 [53] | RT-PCR | POS | Saliva with cough. | – | Higher viral loads were found in saliva samples collected in the morning, showing statistically significant differences when compared to the samples collected at night. |
| Chen et al., 202046 | Cross-sectional study | Patients whose 2019-nCoV nucleic acid detection remained positive before or on the sample collection day. | 31 [15 M, 16 F] | 18-86 [60,6] | RT-PCR | Saliva | Swab at the salivary gland canal opening. | OPS | 13 positive cases for 2019-nCoV nucleic acid detection by oropharyngeal swab (4 cases with positive saliva detection and 3 in serious condition). |
| Nagura-Ikeda et al., 202047 | Cross-sectional study | Patients with laboratory-confirmed COVID-19. | 103 [66 M, 37 F] | 18-87 [46] | LDT RT-qPCR, cobas SARS-CoV-2 test, direct RT-qPCR, RT-LAMP, RAT. | Saliva | Self-collection. | – | The viral RNA detection was significantly higher in saliva samples collected from symptomatic patients within 9 days after the symptoms onset than in samples collected 10 days after symptoms onset or in the saliva from asymptomatic patients. |
| Randad et al., 202048 | Cross-sectional study | Patients with RT-PCR confirmed prior SARS-CoV-2 infection | 28∗ [−] | [−] | RT-PCR, IgG, IgA, IgM | Saliva | Brushing the gum line. | Serum sample. | 22 amostras combinadas obtiveram resultado positivo para detecção de SARS-CoV-2 pela saliva e pelo soro, e 6 resultados foram negativos para ambas amostras. A detecção salivar específica de IgG para SARS-CoV-2 obteve alta sensibilidade e especificidade. |
POS: Posterior Oropharyngeal Saliva; OPS: Oropharyngeal swab; RT-PCR: Reverse Transcription Polymerase Chain Reaction; RT-qPCR: Quantitative Reverse Transcription Polymerase Chain Reaction; LDT: Laboratory developed tests; RT-LAMP: Reverse-Transcription Loop Mediated Isothermal Amplification; RAT: Rapid Antigen Test.
∗
Participants with matched saliva-serum samples.