FIG 2.

(A) Compound 1 and miltefosine activity expressed as 50% inhibitory concentration (IC₅₀) on L. amazonensis (LTB0016 strain) after 48 h of drug exposure against extracellular amastigote forms purified from animal lesion (EF) and intracellular forms (IF) within primary cultures of peritoneal macrophages (PMM), host cell toxicity, and respective selectivity indexes (SI). (B to D) Light microscopy images of Giemsa-stained uninfected (B) and infected PMM (C) subjected or not to 10 μM compound 1 (D), demonstrating parasite sterilization due to drug exposure. Arrows indicate intracellular parasites.