TABLE 4.
Conversion of prodrug 17 to triphosphate in the PBMCs of multiple species
| Compound 17 concn (μM)a | Triphosphate concn (μM) in PBMCs of:b
|
||||
|---|---|---|---|---|---|
| Mouse | Rat | Dog | Monkey | Human | |
| 10 | 8.5 ± 2.5 | ND | 2.8 ± 0.4 | 39.2 ± 14.5 | 15.9 ± 10.6 |
| 100 | 53.4 ± 8.4 | 8.8 ± 0.5 | 13.8 ± 1.8 | 22.6 ± 6.4 | 59.7 ± 19.4 |
a
Compound was incubated in PBMCs at 10 and 100 μM for 24 h, and then the triphosphate concentration was measured by LC-MS/MS.
b
Data are means ± standard deviations (n = 3 biological replicates). ND, not determined.