Figure 4.

SNHG10 Promotes OS Progression through miR-182-5p

(A) The proliferation of OS cells after transfection with sh-SNHG10 or co-transfection with a miR-182-5p mimic was evaluated with a CCK-8 assay. (B) The proliferation of OS cells after transfection with sh-SNHG10 or co-transfection with a miR-182-5p mimic was evaluated using a colony formation assay. (C) The expression of cyclin D1 and E1 in OS cells after transfection with sh-SNHG10 or co-transfection with a miR-182-5p mimic was quantified by western blot. (D) The expressions of E-cadherin, N-cadherin, and vimentin were quantified using western blot after transfection of sh-SNHG10 or co-transfection with a miR-182-5p mimic. (E) The migration of OS cells after transfection with sh-SNHG10 or co-transfection with a miR-182-5p mimic was evaluated with a wound healing assay. (F) The invasion of OS cells after transfection with sh-SNHG10 or co-transfection with a miR-182-5p mimic was evaluated with a transwell assay. In all experiments, bars represent means ± SD from three independent experiments. ∗p < 0.05, ∗∗p < 0.01.