Figure 7.

SNHG10 Activates Wnt/β-Catenin Pathway to Promote OS Progression

(A) β-catenin protein redistribution in different cellular compartments of transfected OS cells was quantified by western blot. (B) β-catenin protein redistribution in different cellular compartments of transfected OS cells was quantified using immunofluorescence staining. Scale bar, 100 μm. (C) Relative expression levels of Wnt/β-catenin pathway downstream target genes were quantified by real-time PCR. (D) Relative TCF/LEF reporter activity was measured after transfection with sh-SNHG10 or co-transfection with FZD3. (E) The β-catenin protein redistribution in different cellular compartments of transfected or XAV-939 treated OS cells was quantified using western blot. In all experiments, bars represent means ± SD from three independent experiments. ∗p < 0.05, ∗∗p < 0.01.