TABLE 1.
Gene functional classification clusters of DEGs between D10 isorhamnetin-treated and D0 control hAECs and D10 isorhamnetin-treated and D10 control hAECs.
| Clusters and top enriched GO in cluster | Enrichment score | Genes in cluster |
| D10 isorhamnetin treated vs. D0 control | ||
| Cluster 1 cilium assembly (GO:0060271), microtubule cytoskeleton organization (GO:0000226), mitotic cell cycle (GO:0000278), cell division (GO:0051301), protein binding (GO:0005515), microtubule binding (GO:0008017) | 13.63 | SPICE1, C1orf116, CEP95, BBOF1, SPATA18, CEP162, CEP55, CEP126, MAP7D3, KIAA0753, FILIP1L, CCDC113, MISP, MTUS2, MZT2A, NREP, RAB3IP, CEP68, CAMSAP2, ZNF365 |
| Cluster 2 cell division (GO:0051301), mitotic cell cycle (GO:0000278), cell population proliferation (GO:0008283), regulation of mitotic spindle organization (GO:0060236), metaphase plate congression (GO:0051310), microtubule binding (GO:0008017), protein kinase binding (GO:0019901), protein binding (GO:0005515) | 9.37 | TPX2, TACC3, SPICE1, CDCA3, PRC1, MISP, MAPRE2, FAM83D, CCNG2, MAP7D3 |
| Cluster 3 cell-cell adhesion (GO:0098609), cell differentiation (GO:0030154), cell junction assembly (GO:0034329), transforming growth factor beta receptor signaling pathway (GO:0007179), zinc ion binding (GO:0008270), cadherin binding involved in cell-cell adhesion (GO:0098641) | 9.17 | FBLIM1, TGFB1I1, PDLIM5, ZYX, PDLIM2, AJUBA, CRIP1, ZNF185, LIMS2, C11orf54, FHL2, FHL1, LMO7, LIMA1, LMCD1, CSRP2, CSRP1, PDLIM1, TES, PRICKLE2, PXN, PDLIM7 |
| Cluster 4 apical constriction (GO:0003383), regulation of cell shape (GO:0008360), integrin-mediated signaling pathway (GO:0007229), transforming growth factor beta receptor signaling pathway (GO:0007179), protein binding (GO:0005515), structural constituent of cytoskeleton (GO:0005200) | 7.54 | FERMT1, RAPH1, FRMD6, FERMT2 |
| Cluster 5 protein ubiquitination (GO:0016567), transcription, DNA-templated (GO:0006351) | 7.51 | FILIP1L, IRF2BP2, CRBN, PRR11 |
| D10 isorhamnetin treated vs. D10 control | ||
| Cluster 1 sequestering of TGFβ in ECM (GO:0035583), positive regulation of cell substrate adhesion (GO:0010811), cell-matrix adhesion (GO:0007160), extracellular matrix organization (GO:0030198), calcium ion binding (GO:0005509), extracellular matrix structural constituent (GO:0005201) | 7.70 | THBS3, SUSD1, EFEMP2, FBN2, FBLN2, NPNT, LTBP2, LTBP1, DNER |
| Cluster 2 O-glycan processing (GO:0016266), G protein-coupled receptor signaling pathway (GO:0007186), cell surface receptor signaling pathway (GO:0007166), cell migration (GO:0016477), cell adhesion (GO:0007155), G protein-coupled receptor activity (GO:0004930) | 7.42 | LGR5, UPK3BL, TMEM40, ALPK2, LY6G6C, GPR85, MYRFL, CLEC7A, TNFRSF25, KLK5, B3GNT3, GPR1, IGFBP7, VTCN1, PSG5, MFAP3L, KERA, BAMBI, TMPRSS11A, MMP23B, SLC39A4, APCDD1, MEST, SLC1A6, CADM1, LRRC32, C1QTNF6, KCNE4, SMCO3, ADGRF5, TRBC1, ADGRF4, SEMA6D, SCARA3, SIGLEC5, B3GNT2, NTM, PAQR5, OPN3, IER3, TSPAN1, GPR155, CD248, RARRES1, IGFBPL1, ADGRF1, GALNT2, ABCG2, DNER, GALNT5, PRRG4, CSF2RB, FAM198B, AMIGO2, GPR37, SUSD1, TMPRSS11B, TMPRSS11E, ACKR3, CXADR, PTPRM, S1PR1 |
| Cluster 3 cell-matrix adhesion (GO:0007160), anatomical structure morphogenesis (GO:0009653), protein binding (GO:0005515), co-receptor activity involved in Wnt signaling pathway, planar cell polarity pathway (GO:1904929) | 7.25 | NTM, LYPD5, GPC4, CPM, MSLN, LY6G6C |
| Cluster 4 extracellular matrix organization (GO:0030198), collagen catabolic process (GO:0030574), extracellular matrix structural constituent (GO:0005201) | 4.80 | EMILIN1, COL1A2, COL1A1, COL4A6 |
| Cluster 5 positive regulation of tyrosine phosphorylation of STAT protein (GO:0042531), positive regulation of peptidyl-tyrosine phosphorylation (GO:0050731), cytokine-mediated signaling pathway (GO:0019221), cytokine receptor activity (GO:0004896) | 4.09 | CSF2RB, GHR, IL6R, IL4R |