KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-brain-derived neurotrophic factor (BDNF) antibody | Santa Cruz Biotechnology | Cat# sc-20981 |
| Rabbit anti-β-actin antibody | Santa Cruz Biotechnology | Cat# NB600–503 |
| Rabbit anti-doublecortin antibody | Abcam | Cat# ab18723 |
| Rabbit anti-β-tubulin antibody | Cell Signaling | Cat# 2128S |
| Ghsr1a mRNA probe | Advanced Cell Diagnostics | Cat# 431991 |
| NeuN mRNA probe | Advanced Cell Diagnostics | Cat# 436351-C2 |
| Dapi mRNA probe | Advanced Cell Diagnostics | Cat# 323108 |
| Rat Ghsr (growth hormone secretagogue receptor) TaqMan probe | Applied Biosystems | Rn00821417_m1 |
| Rat Mchr1 (melanin concentrating hormone receptor 1) TaqMan probe | Applied Biosystems | Rn00755896_m1 |
| Rat Cnr1 (cannabinoid receptor 1) TaqMan probe | Applied Biosystems | Rn00562880_m1 |
| Rat Gapdh (Glyceraldehyde 3-phosphate dehydrogenase) TaqMan probe | Applied Biosystems | Rn01775763_g1 |
| Mouse Ghsr (growth hormone secretagogue receptor) TaqMan probe | Applied Biosystems | Mm00616415_m1 |
| Mouse Gapdh (Glyceraldehyde 3-phosphate dehydrogenase) TaqMan probe | Applied Biosystems | Mm99999915_g1 |
| Bacterial and Virus Strains | ||
| AAV2-GFP-U6-rGHSR-shRNA; shRNA sequence: CCGGACTGCAACCTGGTGTCCTTTGCTCGAGCAAGGACACCAGGTTGCAGTTTTTG |
Vector Biolabs | www.vectorbiolabs.com |
| AAV2-GFP-U6-Scrmb-shRNA | Vector Biolabs | Cat# 7041 |
| AAV-hSyn-ChR2(h134R)-eYFP | Addgene | Cat# 26973-AAV1 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| TaqMan Fast Advanced Master Mix | Applied Biosystems | Cat# 4444557 |
| TaqMan PreAmp Master Mix | ThermoFisher | Cat# 4391128 |
| Ghrelin | Bachem | Cat# 4033076 |
| Cholecystokinin (CCK-8) | Bachem | Cat# 4033010 |
| Critical Commercial Assays | ||
| RNeasy Lipid Tissue Mini Kit | Qiagen | Catalog # 74804 |
| RNeasy Micro Kit | Qiagen | Catalog # 74004 |
| QuantiTect Reverse Transcription Kit | Qiagen | Catalog # 205311 |
| RNAscope® Fluorescent Multiplex Detection Reagent Kit | Advanced Cell Diagnostics | Catalog #320851 |
| Rat Insulin ELISA | Crystal Chem Inc. | Cat #90010 |
| Rat Acetaminophen ELISA | Cambridge Life Sciences | Cat# K8003 |
| Deposited Data | ||
| Data | This paper | https://osf.io/rufcz/ |
| Experimental Models: Organisms/Strains | ||
| Rat: Sprague Dawley (Outbred) | Charles River | N/A |
| Rat: Wistar (Outbred) | Envigo | N/A |
| Mouse: C57BL/6J | Jackson Labs | N/A |
| Rat: Sprague Dawley (Outbred) | Charles River | N/A |