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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: AIDS. 2020 Dec 1;34(15):2211–2221. doi: 10.1097/QAD.0000000000002700

Figure 4. Silencing of HOTAIRM1 or HOXA1 expression in CD33+ cells from HIV-infected subjects abrogates MDSC differentiation and immunosuppressive functions.

Figure 4.

A-B) HOTAIRM1 or HOXA1 expression levels, measured by real-time PCR, in CD33+ cells transfected with control (Con) siRNA, HOTAIRM1 (HOT), or HOXA1 (HOX) siRNA. C) siRNA-mediated knockdown of HOTAIRM1 or HOXA1 expression in CD33+ cells of HIV subjects inhibits CD33+ cell differentiation into MDSCs, determined by flow cytometric analysis of lower HLA-DR− cell frequency. D-F) siRNA-mediated knockdown of HOTAIRM1 or HOXA1 expression in CD33+ cells of HIV subjects inhibits Arg1, iNOS, and STAT3 mRNA expressions, determined by real-time PCR. G-J) siRNA-mediated knockdown of HOTAIRM1 or HOXA1 expression in CD33+ cells of HIV subjects inhibits Arg1, iNOS, and pSTAT3 protein expressions and ROS production, determined by flow cytometry analysis.