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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: J Physiol. 2020 Jun 13;598(15):3085–3105. doi: 10.1113/JP279423

Figure 3. TNF modulates tight junction gene expression by qPCR.

Figure 3.

Real time quantitative PCR (qPCR) was used to examine the relative expression of junction markers A. Claudin-2, B. ZO-1, C. β-catenin, and D. Occludin. HIEs were incubated for 16hours in media, 1 μg/ml IL-1α, TNF and flagellin. The cells were then collected in TRIZOL and RNA isolated. qPCR was then formed to evaluate tight junctions. Data is presented as the ΔΔCT with the house-keeping gene 18S. n=3 wells/experiments, representative of 3 independent experiments. One-Way ANOVA. *p< 0.05.